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. Author manuscript; available in PMC: 2011 Jan 26.
Published in final edited form as: Biochemistry. 2010 Jan 26;49(3):601–610. doi: 10.1021/bi901579y

FIGURE 7.

FIGURE 7

Blue Native polyacrylamide gel electrophoresis of p66 in the absence and presence of NNRTIs. (A) Monomer and homodimer binding to [14C] EFV: (lane 1) 2 μM p66W401A incubated 2 h with [14C] EFV, (lane 2) 5 μM p66 incubated 2 h with [14C] EFV, and (lane 3) 5 μM p66 incubated 1 wk with [14C] EFV. (B) wild-type p66 incubated 1 wk in the absence and presence of NNRTI 1 wk: (lane 1) Native Markers; (lane 2) 0.8 μM p66; (lane 3) 3 μM p66, 25 μM EFV; and (lane 4) 3 μM p66, 25 μM NVP.