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. Author manuscript; available in PMC: 2011 Jan 26.
Published in final edited form as: Biochemistry. 2010 Jan 26;49(3):601–610. doi: 10.1021/bi901579y

Table 2.

Kinetic Parametersa

protein k1b, M–1 s–1 k–1b, 10–5 s–1 Kd(3)app, c, μM k–1(diss)d, 10–5 s–1
p51W401A 13.7 ± 0.7 5.9 ± 0.7 4.2 ± 0.5 9.1 ± 0.2
6.6 ± 0.5f
p66W401A 13.4 ± 0.6 8.1 ± 0.8 6.0 ± 0.3 8.9 ± 0.2
6.6 ± 0.3f
p66/p51 13.3 ± 0.9 6.7 ± 0.9 5.0 ± 0.3 nd
a

RT buffer D, 1mM TCEP, at 5 °C.

b

Calculated from eq 3 by linear regression of data in Figure 5.

c

Kd(3) = k–1/ k1.

d

Calculated from eq 2b. Errors give the range of average values from 2 experiments.