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. Author manuscript; available in PMC: 2011 Jul 15.
Published in final edited form as: Gene. 2010 Apr 2;460(1-2):1–7. doi: 10.1016/j.gene.2010.03.013

Figure 3. The enhancer function of 4E is located in a 565 bp region.

Figure 3

To identify the minimal region required for the enhancer activity of 4E, subfragments of 4E (Figure 1) were subcloned upstream of ADH4 promoter and tested in transient transfections in HepG2 cells (n = 16). Fold change was calculated as ratio of the promoter activity of each construct to the promoter activity of 4 Basal; bars indicate the standard errors of the mean.