Table 3.
Inhibition rate (%) of dithiothreitol (DTT) on the trypsin inhibitory activity of white cloud bean trypsin inhibitor and soybean trypsin inhibitor after incubation at 37°C for different durations.
| Incubation time (min) | Isolated trypsin inhibitor (SP3SU2) | Isolated trypsin inhibitor (SP2SU2) | Soybean trypsin inhibitor | ||||
|---|---|---|---|---|---|---|---|
| 2.5 mM DTT | 10 mM DTT | 40 mM DTT | 2.5 mM DTT | 10 mM DTT | 40 mM DTT | 2.5 mM DTT | |
| 5 | 9.7 ± 1.1a | 24.7 ± 2.5b | 28.8 ± 2.4c | 9.0 ± 1.0a | 22.8 ± 1.9b | 27.0 ± 2.2c | 78.2 ± 5.7a |
| 20 | 30.6 ± 2.9d | 55.0 ± 4.1e | 67.4 ± 4.1f | 28.3 ± 3.0d | 54.1 ± 3.6e | 65.5 ± 4.3f | 93.7 ± 5.0b |
| 80 | 61.7 ± 6.5g | 83.2 ± 5.9h | 94.5 ± 5.6i | 59.6 ± 4.7g | 80.6 ± 6.5h | 91.9 ± 6.6i | 96.4 ± 4.3c |
Results are presented as mean ± SD (n = 3). Different alphabets (e.g., a, b, and c) indicate statistically significant difference (P < .05) when (I) data at same time point and different DTT concentrations or (II) data at same DTT concentration but different time point were analyzed by analysis of variance followed by Duncan's multiple range test.