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Side scatter (SSC) versus GFP fluorescent dot plot of transfected COS-7 cells showing positions of GFP-negative (R1) and GFP-positive (R2) population with the percentage gated of GFP-positive cells using flow cytometry analysis by using different gene carriers. Gene expression was measured at 48 h post-transfection. (a) Untreated, (b) naked DNA, (c) PEI/phMGFP, (d) D-SPM/phMGFP at ratio 12, (e) D-SPM/phMGFP at ratio 14, and (f) D-SPM/phMGFP at ratio16.
