Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Jun 27;2010:376927. doi: 10.1155/2010/376927

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2010 Muriel Roulet et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Selection of clones expressing α1(V) and α2(V) chains. Western blot analysis with antibody to collagen V of recombinant α1(V) and α2(V) chains synthesized and secreted in the culture medium of co-transfected HEK-293 cells. The constructs α1(V)-pZeo-SV2 (lanes 2, 3) or α1(V)-pcDNA-Hygro (lane 4) and the construct α2(V)-Rc-CMV (lanes 2, 3, 4) were co-transfected into the HEK-293 cells. These cells do not naturally express these chains (lane 1). Three clones have been selected to express different ratios of α1(V)/α2(V): clone C22, which expressed more of the α2(V) chain than the α1(V) chain, clone C32, which expressed both chains at approximately the same amount, and clone F22, which expressed more of the α1(V) chain than the α2(V) chain. Left, molecular mass standards expressed in kDa.