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. 2010 Jun 27;2010:376927. doi: 10.1155/2010/376927

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Copyright © 2010 Muriel Roulet et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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(a) Selection of clones expressing proα1(V), proα2(V) chains and HSP47. Western blot analysis of transfected cell media (for proα1(V) and proα2(V) chain detection) or cell lysates (for HSP47 detection) probed with (first panel) 6A7 monoclonal antibody, which recognizes the N-propeptide of the human proα1(V) chain, (second panel) with collagen type V (G-15) antibodies, which recognize the human proα2(V) chain, (third panel) with antibody to HSP47. Of twenty-one clones, only five clones expressed the three partners with different expression levels: C2 (lane 1), C7 (lane 2), C9 (lane3), C13 (lane 4), and C15 (lane 5). The other clones expressed only two of the three partners. For example, clone C12 (lane 6) expressed only the proα1(V) chain and HSP47. (b) Western blot analysis of media from clone C2 treated with (lanes 3, 4) or without monensin (lanes 1, 2) using polyclonal collagen V antibodies. Media were incubated with (lanes 2, 4) or without pepsin (lanes 1, 3). Left, molecular mass standards expressed in kDa.