Figure 7.

Double immunofluorescence staining was performed using monoclonal antibody against human proα1(V) chain (18G5) (a, d, g, j) and rabbit polyclonal proα2(V) chain (b, e, h, m), and merge (c, f, i, l). (a, b, c) human dermal fibroblasts; (d, e, f) clone 22 cells; (g)–(l) HeLa cells transiently transfected with human proα1(V) and proα2(V) constructs. Double immunofluorescence staining shows distinct intracellular distribution of the proα(V) chains in round (g)–(i) and spreading (j, k, l) HeLa cells. Magnification: x1000.