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Example of chromatographic purification of hPRL on SP-Sepharose Fast Flow and SDS-PAGE analysis of CHO cell-conditioned medium of (a) nontreated cells and (b) cells treated with 1 mM NaBu. Lane 1: molecular mass marker. Lane 2: Chemical Reference Standard of G-hPRL and NG-hPRL (WHO-CRS). Lane 3: Internal Reference of periplasmic E. coli-derived hPRL. Lane 4: CHO-conditioned medium. Lane 5: unretained fraction. Lanes 6–8: fractions eluted with 25 mM HEPES, pH 8.0. NaAc: sodium acetate.
