Table 1.
Expression levels and RP-HPLC retention times (tR) of hPRL obtained from CHO cells treated with different concentrations of NaBu for 10 days. The tR were compared with those of the Internal Reference Preparation of periplasmic E. coli-derived hPRL (tR : 26.00 ± 0.72 minutes).
| NaBu (mM) | Expression levels of hPRLa (μg/mL) | tRa (min) |
|---|---|---|
| 0 | 0.92 ± 0.22 | 25.81 ± 0.52 |
| 0.25 | 1.30 ± 0.06 | 25.68 ± 0.47 |
| 0.5 | 1.67 ± 0.00 | 25.90 ± 0.77 |
| 1 | 2.00 ± 0.03 | 25.77 ± 0.73 |
| 2 | 1.48 ± 0.05 | 25.66 ± 0.87 |
| 4 | 1.23 ± 0.11 | 25.68 ± 0.82 |
| Average tR = 25.75 ± 0.09 | ||
| RSD = 0.4% |
aEach value is the mean ± SD of duplicate experiments.