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Recombinant hPRL purification starting from 500 mL of NaBu-treated or untreated CHO-conditioned medium.
| Purification step | Total protein (mg)a | hPRL (mg) | Mass fraction | |||
|---|---|---|---|---|---|---|
| 0 mM NaBu | 1 mM NaBu | 0 mM NaBu | 1 mM NaBu | 0 mM NaBu | 1 mM NaBu | |
| Conditioned medium | 64.7 | 66.2 | 0.54 | 1.20b | 0.008 | 0.018 |
| SP-Sepharose FF | 2.3 | 3.0 | 0.51 | 1.22c | 0.219 | 0.403 |
| Preparative HPSEC | 0.14 | 0.25 | 0.13 | 0.25c | 0.978 | 1.000 |
aEstimated by BCA assay. bEstimated by RP-HPLC. cEstimated by HPSEC.
