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Bioactivity of purified hPRL obtained from CHO cells treated or not with NaBu, determined via Nb2 or Ba/F3-LLP cells against the First International Standard for recombinant hPRL (WHO 97/714) with an activity of 57.2 ± 11.4 IU/mg.
| IU/mg hPRLa | ||
|---|---|---|
| Cells | 0 mM NaBu | 1 mM NaBu |
| Nb2 | 50.88 ± 5.33 | 61.21 ± 4.66 |
| Ba/F3-LLP | 50.07 ± 5.64 | 71.47 ± 4.33 |
aValues are means ± SD of two independent assays, each run with intra-assay quadruplicates.
