Fig. 2.
Intact DHPR tetrad formation with β1aAAA mutant expressed in relaxed myotubes. Freeze-fracture replicas of tail muscle tissue of normal zebrafish larvae (left panel) revealed the arrangement of DHPR particles in tetrads (indicated by red dots) organized in orthogonal arrays. In contrast, the β1-null mutant relaxed (middle panel) lacks DHPR tetrad formation. Comparable to normal larvae, relaxed larvae zygote-injected with in vitro synthesized rb-β1aAAA RNA, displayed correct assembly of DHPR particles in tetradic arrays (right panel). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of the article.)