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. 2010 Jun 3;2010:319192. doi: 10.4061/2010/319192

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Copyright © 2010 Raul A. Almeida et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effect of treatment with host proteins on caveloae-mediated internalization of Streptococcus uberis into bovine mammary epithelial cells. Fibronectin (FN), collagen (Coll), and bovine lactoferrin (LF), pretreated S. uberis UT888 () and UT366 () were cocultured with bovine mammary epithelial cells treated with the receptor-mediated endocytosis inhibitor monodansylcadaverine (MDC) and colony forming units per ml (cfu/mL) of intracellular S. uberis calculated. Data are presented as the percentage of untreated controls (100%; UT888 = 7.9 × 10⁴; UT366 = 7.0 × 10³) and bars represent the standard error of the mean (SEM) of three independent experiments run in triplicate. (∗) indicates statistically significant differences (P < .05) with the corresponding control. Means with similar superscript letters within each strain are statistically different (P < .05).

Inline graphic — Effect of treatment with host proteins on caveloae-mediated internalization of Streptococcus uberis into bovine mammary epithelial cells. Fibronectin (FN), collagen (Coll), and bovine lactoferrin (LF), pretreated S. uberis UT888 () and UT366 () were cocultured with bovine mammary epithelial cells treated with the receptor-mediated endocytosis inhibitor monodansylcadaverine (MDC) and colony forming units per ml (cfu/mL) of intracellular S. uberis calculated. Data are presented as the percentage of untreated controls (100%; UT888 = 7.9 × 10⁴; UT366 = 7.0 × 10³) and bars represent the standard error of the mean (SEM) of three independent experiments run in triplicate. (∗) indicates statistically significant differences (P < .05) with the corresponding control. Means with similar superscript letters within each strain are statistically different (P < .05).