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. 2010 Jun 3;2010:319192. doi: 10.4061/2010/319192

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Copyright © 2010 Raul A. Almeida et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Transcytosis of Streptococcus uberis pretreated with host proteins across bovine mammary epithelial cells treated with the receptor mediated endocytosis inhibitor monodansylcadaverine detected at different incubation times. Fibronectin (FN), collagen (Coll), or bovine lactoferrin (LF) pre-treated S. uberis UT888 was cocultured with bovine mammary epithelial cells treated with the receptor-mediated endocytosis inhibitor monodansylcadaverine (MDC), and colony forming units per ml (cfu/mL) of S. uberis that transcytose bovine mammary epithelial cells were calculated at 60 (), 120 (), and 180 () min of incubation. Data are presented as the percentage of transcytosis through monodansylcadaverine treated bovine mammary epithelial cells (100%, 60 minutes = 5.1 × 10², 120 minutes = 6.3 × 10²; 180 minutes = 1.0 × 10³) at each incubation time and bars represent the standard error of the mean of three independent experiments run in triplicate. (∗) indicates statistically significant differences (P < .05) with the corresponding control. Means with similar superscript letters within each sampling time are statistically different (P < .05).

Inline graphic — Transcytosis of Streptococcus uberis pretreated with host proteins across bovine mammary epithelial cells treated with the receptor mediated endocytosis inhibitor monodansylcadaverine detected at different incubation times. Fibronectin (FN), collagen (Coll), or bovine lactoferrin (LF) pre-treated S. uberis UT888 was cocultured with bovine mammary epithelial cells treated with the receptor-mediated endocytosis inhibitor monodansylcadaverine (MDC), and colony forming units per ml (cfu/mL) of S. uberis that transcytose bovine mammary epithelial cells were calculated at 60 (), 120 (), and 180 () min of incubation. Data are presented as the percentage of transcytosis through monodansylcadaverine treated bovine mammary epithelial cells (100%, 60 minutes = 5.1 × 10², 120 minutes = 6.3 × 10²; 180 minutes = 1.0 × 10³) at each incubation time and bars represent the standard error of the mean of three independent experiments run in triplicate. (∗) indicates statistically significant differences (P < .05) with the corresponding control. Means with similar superscript letters within each sampling time are statistically different (P < .05).