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. Author manuscript; available in PMC: 2010 Jul 5.
Published in final edited form as: Chem Biol Drug Des. 2007 Jan;69(1):31–40. doi: 10.1111/j.1747-0285.2007.00463.x

Figure 5.

Figure 5

FRET between NTA2-BM and FlAsH. (A) Fluorescence emission spectra of His10-CRABP I St1-2* either FlAsH-labeled (dashed line) or doubly labeled (with both FlAsH-EDT2 and NTA2-BM; solid line). An increase in the FlAsH fluorescence in the doubly-labeled protein arises from energy transfer from donor, bimane, to acceptor, FlAsH. (B) Subjecting the doubly-labeled protein to trypsinolysis resulted in an increase in the bimane (donor) fluorescence of the trypsin-treated protein compared with that of the untreated protein due to the loss of the acceptor.