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C12orf65 Does Not Have Detectable Peptidyl-tRNA Hydrolase Activity in an In Vitro Assay with Bacterial Ribosomes
E. coli ribosomes were programmed with f[3H]-Met tRNAMet at the P site and the indicated codons at the A site. Activity was measured as specific hydrolysis of f[3H]-Met from its cognate tRNAMet and expressed as cpm released. mtRF1a/mtRF1L, which recognizes the two mitochondrial termination codons UAA and UAG, was used as a positive control.
