Figure 5.

Expression Patterns of SULF1 and SLCO5A1 in a Panel of Human Tissues

cDNA were obtained via the MMLV reverse transcriptase (Invitrogen) with random primers from 1 μg of human total RNA of five adult and four fetal tissues and two cultured cells (osteoblasts and osteoclasts). Real-time PCR was performed in triplicate with Takara SYBR premix on Light Cycler 480 (Roche diagnostics). The ΔΔCt method¹¹ was used to assess expression level of two target genes—SULF1 (A) and SLCO5A1 (B)—relative to the expression level of the GAPDH housekeeping gene. For a given target gene, the ΔCt of each tissue was compared to the median of the ΔCt of the 11 tissues analyzed. As a control, we assessed the HPRT1 housekeeping gene expression in the different tissues with the same method and showed minor differences between HPRT1 and GAPDH genes (C).