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. Author manuscript; available in PMC: 2010 Jul 5.
Published in final edited form as: Oncogene. 2009 Nov 30;29(9):1293–1302. doi: 10.1038/onc.2009.420

Figure 3.

Figure 3

Macroscopic and microscopic examination of orthotopic prostate tumors and organs with metastasis lesions. (a) Macroscopic appearance of selected urogenital organs from animals implanted with PC-3-MIC-1 and PC-3-vector cells. Seminal vesicles and prostate tumor were dissected from 40-day-old animals from both groups. There was no difference in the gross appearance of tumors derived from both groups. But, animals injected with PC-3-MIC-1 cells were found to be very week, emaciated and having less body weight than animals injected with PC-3-vctor control cells. (b) Immunohistochemical analysis of primary tumors developed in immunodeficient mice by orthotopic implantation of PC-3-MIC-1 and PC-3-vector cells. Tumor sections were stained for macrophage inhibitory cytokine-1 (MIC-1)-positive cells using MIC-1-specific affinity-purified anti-rabbit polyclonal antibody. The two panels represent × 10 and × 20 magnification. Tissue derived from PC-3-MIC-1 tumors shows high signal intensity. (c) Histological appearance of liver, lung and lymph node showing prominent metastasis in the mice implanted with PC-3-MIC-1 cells. Normal (N) and cancer cells (Ca) in different organs are separately indicated, but in the lymph node, the normal tissues are completely occupied by tumor cells. It was found that MIC-1 promotes metastasis in the orthotopic prostate cancer model.