Effect of 1α,25 (OH)2D3 treatment on MG63 cell differentiation. MG63 cells were cultured on TCPS, PT, SLA, and modSLA Ti surfaces. At confluence, cultures were treated with 10-9M or 10-8M 1α,25 (OH)2D3 for 24 hours. (A) Osteocalcin and (B) VEGF-A levels in the conditioned medium were determined. Values are mean ± SEM of six independent cultures. The data presented are from one of two experiments with comparable results. Data were analyzed using ANOVA and statistical significance between groups was determined using Bonferroni's modification of Student's t-test. *p< 0.05 vs. TCPS; ·p<0.05 vs. PT; Φp<0.05 vs. SLA.