Table 2.
Immunophenotype evaluation of transfected MSC through flow cytometry analysis. The identification of MSC characteristic phenotype was assessed by fluorescent-conjugated monoclonal antibodies against CD73, CD105, and CD90 receptors. Controls with non-transfected cells were also performed as well as the appropriate isotype controls IgG γ1 and IgG γ2.
Phenotype marker | CD73+ | CD105+ | CD90+ |
---|---|---|---|
MSC (control) | 96.5% | 95.3% | 92.1% |
Transfected MSC | 89.3% | 87.0% | 89.8% |
GFP+ MSC | 83.0% | 74.2% | 86.5% |