Table 2.

Immunophenotype evaluation of transfected MSC through flow cytometry analysis. The identification of MSC characteristic phenotype was assessed by fluorescent-conjugated monoclonal antibodies against CD73, CD105, and CD90 receptors. Controls with non-transfected cells were also performed as well as the appropriate isotype controls IgG γ1 and IgG γ2.

Phenotype marker	CD73+	CD105+	CD90+
MSC (control)	96.5%	95.3%	92.1%
Transfected MSC	89.3%	87.0%	89.8%
GFP+ MSC	83.0%	74.2%	86.5%