Figure 4.

(a) The peptide with m/z approximately 3790 was identified as the fragment of prothymosin alpha by the following MS/MS microsequencing. (b) Mass spectrum of proteins pulled out from cytoplasmic fraction of murine macrophage samples by antiprothymosin alpha which was coupled to protein G agarose beads (mass range from 1000 to 20,000 m/z). Antiprothymosin alpha-coupled protein G agarose beads were incubated with cytoplasmic fraction and eluted proteins were analyzed on NP 20 ProteinChip array.