Figure 5.

(a) Induction of (pro)thymosin-α expression by PICV infection. P388D1 cells were infected with either P2 or P18 PICV in triplicate and tissue culture supernatants collected at various times post infection for determination of (pro)thymosin-α levels by ELISA. Fold increases are compared to levels from triplicate samples at time = 0 and show the results from three experiments. Error bars show the standard error of the mean, and asterisks indicate significant (P < .05) differences in levels induced between P2 and P18 infection as determined by Student's t-test. (b) Changes in stathmin expression following infection by P2 and P18 PICV. Triplicate cultures of P388D1 cells were infected with PICV and whole cell lysates assayed or stathmin levels by immunoblotting.