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. 2010 Jun 29;2010:970491. doi: 10.1155/2010/970491

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Copyright © 2010 S. E. Goñi et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Western Blotting with α-Tio-Z-V5-His. (a) The ultracentrifugated supernatant (UC pellet) and cellular extract (Cell) of Sf9 cell fractions infected with recombinant v_Ac-Z were loaded in lanes 1 and 2. The same fractions were loaded for uninfected cells (ui, lanes 3 and 4) and control v_dsRed (recombinant baculovirus expressing dsRed protein, lanes 5 and 6). (b) Proteinase K protection assay. The treatment of the UC pellet samples (Triton X-100 1% and Proteinase K 0,1 mg/ml) is indicated on the top of the figure.