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. 2010 Jun 29;2010:970491. doi: 10.1155/2010/970491

Table 1.

Biochemical properties of the different expressed proteins.

Protein Number of amino acids MW (Da) pI Extinction coefficients1
Z 106 11560.1 8.44 15970/15470
Tio-Z-V5-His 251 27339.1 6.29 30075/29450
Tio 120 12814.5 4.71 14105/13980
Z-V5-His 131 14542.5 8.43 15970/15470
GST-Z 331 37855.6 6.79 59080/58330
GST 225 26293.2 6.00 43110/42860
His-Z 136 15432.4 7.09 21930/21430

1Calculated extinction coefficients in M−1 cm−1 units, measured to 280 nm in water. Left numbers correspond to the calculation assuming that all Cys residues appear as half cystines, while the right numbers correspond to the calculation assuming that no Cys residues appear as half cystines.