Table 1.
Protein | Number of amino acids | MW (Da) | pI | Extinction coefficients1 |
---|---|---|---|---|
Z′ | 106 | 11560.1 | 8.44 | 15970/15470 |
Tio-Z-V5-His | 251 | 27339.1 | 6.29 | 30075/29450 |
Tio | 120 | 12814.5 | 4.71 | 14105/13980 |
Z-V5-His | 131 | 14542.5 | 8.43 | 15970/15470 |
GST-Z | 331 | 37855.6 | 6.79 | 59080/58330 |
GST | 225 | 26293.2 | 6.00 | 43110/42860 |
His-Z | 136 | 15432.4 | 7.09 | 21930/21430 |
1Calculated extinction coefficients in M−1 cm−1 units, measured to 280 nm in water. Left numbers correspond to the calculation assuming that all Cys residues appear as half cystines, while the right numbers correspond to the calculation assuming that no Cys residues appear as half cystines.