Figure 2.

Epitope mapping of mAb 1A11 C1 using direct ELISA. Synthetic peptides corresponding to the complete NiV N protein sequence (a.a. 1-532) were serial diluted and coated at concentration from 1 μg to 0.1 ng per well (100 μL in volume). A peptide from Alkhurma virus E protein (Alk E, a.a. 143-168) was included as negative control and signal cutoff value (0.17) was calculated based on readings from this peptide. NiV infected Vero lysate diluted 10 fold to 10⁵ fold were served as positive control.