Table 1.
SSN | Location | Primers | Restriction enzyme and sizes (bp) |
---|---|---|---|
SSN10 | GTF2IRD2 Intron 4 |
Forward: 5′-TGCAAGGTCGTAATTCTCAGG-3′ Reverse: 5′-TGTCTTTCCATAGGCATGAAGA-3′ |
MspI Bt: 42; 201; 753 Bc+Bm: 42; 954 |
SSN11 | GTF2IRD2 Intron 3 |
Forward: 5′-TTGTAAAATGGTGTTTATTTTAGG-3′ Reverse: 5′-GCCCCACAAACTTGGATCTG-3′ |
Tru9I Bt: 20; 51; 242; 156 Bc+Bm: 20; 51; 100; 142; 156 |