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. Author manuscript; available in PMC: 2010 Jul 5.
Published in final edited form as: Biochemistry. 2005 Feb 8;44(5):1550–1560. doi: 10.1021/bi048089z

Figure 4.

Figure 4

Effects of charge neutralization on receptor-coupled kinase activity in vitro. Receptors were expressed in a strain lacking all soluble pathway components. Native membranes were isolated, and the ability of each modified receptor to activate the kinase in the reconstituted ternary complex was assessed. Each bar represents the observed receptor-coupled kinase activity in the presence (white bars) and absence (black bars) of aspartate, normalized to wild-type kinase activity in the absence of aspartate. Reaction mixtures at 22 °C contained 2 μM receptor monomer, 2 μM CheW monomer, 0.5 μM CheA monomer, and 10 μM CheY in a buffer containing 50 mM Tris-HCl (pH 7.5), 50 mM KCl, 5 mM MgCl2, and 100 μM ATP.