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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Cell Microbiol. 2010 Jan 20;12(7):919–929. doi: 10.1111/j.1462-5822.2010.01440.x

Figure 3. Chlorpromazine and monodansyl cadaverine block EspF/SNX9-induced membrane tubulation.

Figure 3

HeLa cells co-transfected with EGFP-EspF and mCherry-SNX9 were incubated with DMEM (Untreated), or DMEM with 0.1% DMSO, 60μM chlorpromazine (CPZ), 80μM dynasore, 10mM methyl-beta-cyclodextrin (MBCD), or 400μM monodansyl cadaverine (MDC). Inhibitor treatment did not affect expression levels of either transgene. Both EGFP-EspF and mCherry-SNX9 localized to tubules in all conditions except treatment with CPZ and MDC, in which case both proteins exhibited a punctate staining pattern. Images are representative of three independent experiments.