FIG. 2.

Scoring system for rescued embryos. The level of gata1 rescue was determined by the percentage of rescued mutant embryos in each well and by the intensity of gata1 expression in these embryos in comparison to the negative and positive control wells for each individual plate. DMSO vehicle was the negative control, and 20 μM DEAB was the positive control. Representative mutant embryos are shown after in situ hybridization for gata1 and cdx4 expression. Color images available online at www.liebertonline.com/zeb.