Figure 2.

The effect of treatment of erythrocytes during storage on the capacity to bind autologous IgG.

Erythrocytes of various storage periods were subjected to incubation with hyperosmotic medium inducing cell shrinkage, with hydroperoxide inducing oxidation, with ionomycin inducing an increase in intracellular calcium concentration, and with SITS inducing crosslinking of band 3 molecules. After these treatments the cells were incubated with autologous plasma and IgG binding was determined by flowcytometry using FITC-labeled anti-human IgG (Cappel). Hyperosmotic shock and SITS induce an increase in IgG binding starting at three weeks of storage.