TABLE 1.
Complement-mediated serum bactericidal antibody responses of mice immunized with fHbp vaccines
| Studya | Mouse strain | No. of doses | fHbp vaccine |
|||
|---|---|---|---|---|---|---|
| WT |
E218A/E239A mutant |
|||||
| 1/mean log10 titer ± 2 SE | 1/GMT | 1/mean log10 titer ± 2 SE | 1/GMT | |||
| 1 | CD-1 | 3 | 2.63 ± 0.54 | 427 | 2.31 ± 0.25 | 206 |
| 2 | CD-1 | 1 | 1.26 ± 0.36 | 18b | 0.80 ± 0.14 | 6b |
| 3 | BALB/c | 3 | 3.30 ± 0.30 | 1,986b | 1.68 ± 0.60 | 48b |
a
In study 1, the sera were pooled (3 pools per vaccine group, each pool from sera of 3 to 4 mice). The respective GMTs between vaccine groups were not significantly different. In studies 2 and 3, individual sera were assayed (n = 6 to 8 mice per vaccine group) and the respective differences were significant for both studies. Study 2 also used different lots of WT and mutant fHbp vaccines than those used in study 1 or 3.
b
P was <0.05 for comparison of the respective WT and mutant vaccine groups in each study.