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Western blot analysis of recombinant syndecan-Fc hybrid molecules. Protein A-purified Fc (1 μg) (lane 1), syndecan-1-Fc (1 μg) (lane 2), syndecan-1-Fc (1 μg) pretreated for 2 h at 37°C with a mixture of heparinases I, II and III (10 U) (lane 3), and the heparan sulfate chainless syndecan-1-Fc mutant called Δ3HS-syndecan-1-Fc (lane 4) (1 μg) were analyzed by Western blotting using anti-human Fc IgG (1/1,000 dilution). Fc and syndecan-1-Fc proteins (bands) are boxed.
