TABLE 1.
Syndecan-1-Fc exhibits a broad anti-HIV spectrum
| Virus and/or isolate | Clade | Coreceptor usage | Syndecan-1-Fc activity (μg/ml) |
Fc control activity (μg/ml) |
||
|---|---|---|---|---|---|---|
| IC50 | IC90 | IC50 | IC90 | |||
| HIV-1 group M isolatesa | ||||||
| 92RW021 | A | R5 | 1.9 ± 0.06 | 6.1 ± 0.2 | >100 | >100 |
| 92UG029 | A | X4 | 2.2 ± 0.08 | 8.2 ± 0.07 | >100 | >100 |
| 92TH026 | B | R5 | 0.3 ± 0.02 | 4.9 ± 0.11 | >100 | >100 |
| 92HT599 | B | X4 | 2.1 ± 0.11 | 7.7 ± 0.23 | >100 | >100 |
| 93IN101 | C | R5 | 1.2 ± 0.04 | 4.6 ± 0.14 | >100 | >100 |
| 98IN017 | C | X4 | 1.6 ± 0.1 | 3.6 ± 0.06 | >100 | >100 |
| 92UG005 | D | R5 | 1.9 ± 0.03 | 9.3 ± 0.14 | >100 | >100 |
| 92UG024 | D | X4 | 0.8 ± 0.05 | 7.8 ± 0.06 | >100 | >100 |
| 92TH006 | E | R5 | 2.4 ± 0.12 | 4.5 ± 0.23 | >100 | >100 |
| 93TH053 | E | X4 | 2.3 ± 0.11 | 7.4 ± 0.19 | >100 | >100 |
| 93BR029 | F | R5 | 2.1 ± 0.06 | 6.3 ± 0.07 | >100 | >100 |
| 93BR020 | F | X4 | 1.0 ± 0.02 | 4.9 ± 0.25 | >100 | >100 |
| RU132 | G | R5 | 0.4 ± 0.06 | 3.3 ± 0.03 | >100 | >100 |
| Jv1083 | G | R5 | 2.4 +/− 0.13 | 7.2 ± 0.17 | >100 | >100 |
| Drug-resistant HIV-1 virusesa | ||||||
| RT74V | 2.4 ± 0.14 | 9.1 ± 0.24 | >100 | >100 | ||
| RTM184V | 0.8 ± 0.03 | 5.4 ± 0.07 | >100 | >100 | ||
| PRM46I/L63P/V82T/I84V | 0.5 ± 0.01 | 3.9 ± 0.08 | >100 | >100 | ||
| PRG48V/L90M | 1.1 ± 0.03 | 7.4 ± 0.13 | >100 | >100 | ||
| T20V38A/N42D | 0.6 ± 0.07 | 5.6 ± 0.04 | >100 | >100 | ||
| T20N425 | 1.4 ± 0.1 | 8.3 ± 0.5 | >100 | >100 | ||
| Other retroviruses | ||||||
| SIVmac251 43H | 1.7 ± 0.04 | 6.9 ± 0.3 | >100 | >100 | ||
| SIVsyk1.2 | 1.3 ± 0.02 | 5.4 ± 0.16 | >100 | >100 | ||
| HIV-2CDC310342 | 2.3 ± 0.11 | 8.6 ± 0.26 | >100 | >100 | ||
| HIV-27312A | 2.9 ± 0.04 | 9.2 ± 0.05 | >100 | >100 | ||
a
Representative of various HIV-1 subtypes with different coreceptor usage.
b
See Materials and Methods for a description of the viruses.