TABLE 1.
Strains and primers used in this study
| Strain or primer specificity and primer | Genotype, phenotype, or sequencea | Reference or source |
|---|---|---|
| Strains | ||
| Staphylococcus epidermidis | ||
| 9142 | Wild-type PIA-producing strain | 19 |
| CAV1005 | Native valve endocarditis clinical isolate | 37 |
| 5179 | CSF shunt infection isolate; biofilm forming- and PNAG-negative strain, icaA::IS257 | 30 |
| 5179-R1 | Strain 5179 revertant that produces a proteinaceous biofilm dependent on a truncated 140-kDa isoform of Aap, PNAG negative, icaA::IS257 | 30 |
| ICS1 | CRBSI | This study |
| ICS2 | CRBSI | This study |
| ICS3 | CRBSI | This study |
| ICS4 | CRBSI | This study |
| ICS5 | CRBSI | This study |
| Staphylococcus aureus NCTC 8325 | Reference strain | 12 |
| Staphylococcus hominis subsp. novobiosepticus F13532 | Blood culture isolate | 2 |
| Staphylococcus haemolyticus S33208 | Blood culture isolate | This study |
| Primer (5′ → 3′) | ||
| serp0107 | ||
| Serp0107F | TTGAGCTTGTCATTGGTTCG | 16 |
| Serp0107R | TGTAGAGGTTGCACGTCGAG | 16 |
| tuf | ||
| TStaG422 | GGCCGTGTTGAACGTGGTCAAATCA | 21 |
| TStag765 | TIACCATTTCAGTACCTTCTGGTAA | 21 |