Value of the pfmdr1 copy number in isolates from West Africa and Central Africa. A total of 131 isolates were collected in 2005 to 2009 (2005 [n = 5], 2006 [n = 35], 2007 [n = 33], 2008 [n = 34], and 2009 [n = 24]). From 131 isolates, only 4 had pfmdr1 copy number amplification. The other 127 isolates had a mean pfmdr1 copy number value of 1.08 ± 0.14. Of these 131 isolates, 99 isolates were from West Africa (38 from Ivory Coast, 18 from Burkina Faso, 10 from Senegal, 9 from Guinea Conakry, 6 from Mali, 6 from Benin, 4 from Togo, 3 from Mauritania, 2 from Nigeria, 2 from Ghana, and 1 from Liberia) and 32 isolates were from Central Africa (17 from Cameroon, 8 from the Central Africa Republic, 4 from Gabon, 2 from Congo, and 1 from Chad). Each symbol shows the pfmdr1 copy number for one isolate (mean of three replicate assays).