TABLE 2.
Construction of plasmids used in this study
| Figure | Product | Primer pair | Cloning vector |
|---|---|---|---|
| 1B | p2UY21-LuxR | 44122038-44122037 | p2UY21 |
| 1, 2 | pAH125-PexsC | 39530603-49188917 | pAH125 |
| 2 | pGEX-rpoD (K593A, R596A, R599A) | 48669731-48669730 | pGEX-rpoD |
| 3-6 | RpoDHis | 43812190-43812191 | pET-23b |
| 3 | RpoD (K597A) | 46001014-46001013 | pET23RpoDHis |
| 3 | RpoD (R603A) | 47437714-47437715 | pET23RpoDHis |
| 3 | RpoD (D616A) | 47437713-47437712 | pET23RpoDHis |
| 3 | RpoD (K597A,R603A) | 46001014-46001013 | pET23RpoDHis (R603A) |
| 3 | RpoD(K597A, R600A, R603A) | 48432036-48432035 | pET23RpoDHis (K597A,R603) |
| 3-6 | RpoAHisCTD | 46775590-46775589 | pET-24a |
| 3-6 | RpoB | 46775588-46775587 | pET-24a |
| 3-6 | RpoC | 47100507-46775585 | pET-24a |
| 3 | pOM90-PexsC | 35048925-35048926 | pOM90 |
| 4 | PexsC (G41T) | 43648443-43648442 | pSA508-PexsC |
| 4 | PexsC (T40A) | 48552525-48552524 | pSA508-PexsC |
| 4 | PexsC (G39C) | 48552527-48552526 | pSA508-PexsC |
| 4 | PexsC (A38T) | 43648441-43648440 | pSA508-PexsC |
| 4 | PexsC (C37G) | 43648439-43648438 | pSA508-PexsC |
| 4 | PexsC (A36T) | 48552529-48552528 | pSA508-PexsC |
| 4 | PexsC (A33G) | 43648437-43648436 | pSA508-PexsC |
| 4-6 | PexsC (TG) | 48552531-48552530 | pSA508-PexsC |
| 4-6 | PexsC (T8G) | 43579324-43579323 | pSA508-PexsC |
| 6 | pOM90-Ptrc(250) | 25444818-25444816 | pOM90 |
| 6 | pOM90-Ptrc(179) | 25444818-25444814 | pOM90 |
| 6 | pOM90-PRE# | 48495914-48495913 | pOM90 |
| 6 | RpoD (1-574) | 43812190-48495915 | pET-23b |