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. 2010 May 14;192(14):3639–3644. doi: 10.1128/JB.00358-10

FIG. 1.

FIG. 1.

Loss of RpoS reduces expression of the dinB gene in stationary-phase cells only. β-Galactosidase assays were performed as previously described (29). The graphs on the left show the results of β-galactosidase assays of four independent isolates of each strain grown to an OD of ∼0.2 in M9 plus glycerol minimal medium. The graphs on the right show the results of β-galactosidase assays of four independent isolates of each strain grown for 48 h to saturation in M9 plus glycerol minimal medium (see Materials and Methods). Error bars are standard errors of the means (SEM). (A) Strains carried a translational fusion of the first 6 amino acids of dinB fused in frame to the lacZ gene and were grown at 37°C. Wild type, PFB505; rpoS::Cm, PFB593. (B) Strains carried the dinB::Mud(Apr lac) transcriptional fusion described previously (19) and were grown at 30°C. Wild type, GW1030; rpoS::Cm, PFB893.