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Both EσS and Eσ70 bind to the dinB promoter. A 214-bp region containing the promoter of the dinB gene and labeled with an infrared dye, IRdye 700 (Li-Cor Biosciences), was incubated with RNA polymerase holoenzyme containing either σS or σ70. The reaction mixture was electrophoresed on a nondenaturing polyacrylamide gel and visualized with an Odyssey IR scanner.
