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Loss of RpoS increases cell sensitivity to 4-NQO whether or not Pol IV is overexpressed from an exogenous promoter. Spot tests measuring sensitivity to 10 μM 4-NQO were performed as described in Materials and Methods; only the 10−5 and 10−6 dilutions from the untreated LB plate are shown. Wild type, FC722; rpoS::Cm, PFG419; wild-type/pdinB++, PFG266; rpoS::Cm/pdinB++, PFG420.
