TABLE 1.
E. coli strains and plasmid used in this study
| Strain or plasmid | Relevant phenotype or genotype | Reference |
|---|---|---|
| E. coli strains | ||
| FC36 | F−ara Δ(lac-proB)XIIIthi Rifr | 4 |
| FC40 | FC36/F′ φ(lacI33-lacZ) Pro+ | 4 |
| FC722 | FC40 with a Tcs allele on the episome | 9 |
| FC1413 | FC942 sulA11 lexA71::Tn5 | 25 |
| PFG266 | FC722 with pPFG96 | 38 |
| PFG419 | FC722 rpoS::Cm | This study |
| PFG420 | PFG419 with pPFG96 | This study |
| PFB505 | FC1413 dinB-lacZ translational fusion in λ att | This study |
| PFB593 | PFB505 rpoS::Cm | This study |
| PFB691 | FC36 with pPFV323 | This study |
| GW1030 | AB1157 recA441 sulA1 ΔlacUI69 dinB1::Mud(Ap lac) | 19 |
| PFB893 | GW1030 rpoS::Cm | This study |
| Plasmids | ||
| pPFG96 | dinB in pBAD24 | 38 |
| pBAD24 | Cloning vector with a pBR322 origin and araBAD promoter | 14 |
| pPFV323 | dinB-lacZ operon fusion in pRS415 | This study |
| pRS415 | lacZ operon fusion vector | 36 |
| pMC1403 | lacZ gene fusion vector | 5 |