QseD regulates the qseD operon and surrounding genes in E. coli. qRT-PCR of the qseD operon (iadA, yjiG, and yjiH) and the surrounding genes (kptA, yjiC, and yjiD) in EHEC 86-24, the ΔqseD mutant, the ΔqseD mutant complemented in trans with qseD (86-24), and the ΔqseD mutant complemented in trans with qseD (K-12) grown in DMEM (OD600 of 1.0).