TABLE 1.
Oligonucleotide sequences used to create structural duplex substratesa
| Primer or template | Length (nt) | Sequence |
|---|---|---|
| Primers | ||
| 1 | 32 | ugccaagcuugcaugccugcaggucgacucua |
| 2 | 30 | auucguaaucauGGTCATAGCTGTTTCCTG |
| 3 | 17 | TGCCAAGCTTGCATGCC |
| 4 | 37 | TGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGA |
| 5 | 12 | TGGGTGGGGTGG |
| 6 | 30 | ATTCGTAATCAuGGTCATAGCTGTTTCCTG |
| Templates | ||
| 7 | 87 | CAGGAAACAGCTATGACCATGATTACGAATTCGAGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTGGCA |
| 8 | 30 | CAGGAAACAGCTATGACCATGATTACGAAT |
| 9 | 30 | CAGGAAACAGCTATGACCCCACCCCACCCA |
| 10 | 30 | CAGGAAACAGCTATGACCAGGATTACGAAT |
| 11 | 30 | CAGGAAACAGCTATGACCGTGATTACGAAT |
| 12 | 30 | CAGGAAACAGCTATGACTATGATTACGAAT |
The S1 substrate comprises primer 2 and template 8, the S2 substrate is primer 2, the S3 substrate comprises primers 2 and template 8, the S4 substrate consists of primers 2 and 3 and template 7, the S5 substrate contains primers 2 and 4 and template 7, the S6 substrate includes primers 2 and 5 and template 9, the S7 substrate consists of primer 2 and template 12, the S8 substrate is composed of primer 2 and template 11, the S9 substrate consists of primer 2 and template 10, the S10 substrate comprises primer 6 and template 8, and the S11 substrate contains primer 1 and template 7. Deoxyribonucleotides and ribonucleotides are shown by uppercase and lowercase letters, respectively.