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Electrophoretic mobility shift assays demonstrate a direct interaction of PypB with the pypB and flp promoter region. Increasing amounts of recombinant PypBΔc were incubated with a DIG-labeled DNA fragment encompassing 500 nt upstream of the pypB start codon (A) and 250 bp upstream of the flp start codon (B), resulting in a mobility shift of the DNA fragment. Shifting of the probe can be inhibited by specific but not by unspecific unlabeled competitor DNA.
