TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristic(s) | Reference or source |
|---|---|---|
| Strains | ||
| Y. enterocolitica | ||
| JB580v | ΔyenR (r− m+) Nalr; serogroup O:8 | 19 |
| GHY545 | JB580v Δflp | This study |
| E. coli | ||
| DH5α | φ80dΔ(lacZ)M15 Δ(argF-lac)U169 endA1 recA1 hsdR17(rK− mK+)deoR thi-1 supE44 gyrA96 relA1 | Gibco BRL |
| S17-1λpir | Tpr SmrrecA thi pro hsdR M+ RP4::2-Tc::Mu::Km Tn7λpir lysogen | 26 |
| Plasmids | ||
| pEP185.2 | Camrmob+ (RP4) R6K ori (suicide vector) | 19 |
| pKW1 | Ampr Strep/Specr; low-copy-no. lacZYA reporter plasmid | 38 |
| pBAD18Kan | Kanr; PBAD expression vector | 11 |
| pBAD33 | Camr; PBAD expression vector | 11 |
| pWSK129 | Kanr; low-copy-no. cloning vector | 39 |
| pET-pypBΔc | PypBΔc expression vector | 38 |
| pEP-Δflp | Camr; flp deletion construct | This study |
| pKW-flp150 | flp-lacZYA transcriptional fusion containing 150 nt 5′ of flp start codon | This study |
| pKW-flp250 | flp-lacZYA transcriptional fusion containing 250 nt 5′ of flp start codon | This study |
| pKW-flp500 | flp-lacZYA transcriptional fusion containing 500 nt 5′ of flp start codon | This study |
| pKW-pypB126 | pypB-lacZYA transcriptional fusion containing 126 nt 5′ of pypB start codon | This study |
| pKW-pypB244 | pypB-lacZYA transcriptional fusion containing 244 nt 5′ of pypB start codon | This study |
| pKW-pypB500 | pypB-lacZYA transcriptional fusion containing 500 nt 5′ of pypB start codon | This study |
| pBAD-pypB | pypB in pBAD18Kan | 38 |
| pBAD33-pypB | pypB in pBAD33 | This study |
| pWSK-pypB/flpHA | pypB and flp under the control of their native promoters in pWSK129; expression of Flp with a C-terminal HA tag | This study |
| pWSK-kflp | pypB and flp under the control of their native promoters in pWSK129 | This study |