TABLE 2.
Primers used in this study
| Oligonucleotide | Sequence (5′→3′)a | Restriction site |
|---|---|---|
| JS-pypB126 | GCTCTAGACACGCACACAACATCAAAGAG | XbaI |
| JS-pypB244 | GCTCTAGACATCATGCAAGAATACGTCG | XbaI |
| JS-pypB1 | GCTCTAGAGCCGTTGCATCACTAAGACTG | XbaI |
| JS-pypB-200r | CGGAATTCTTCACTGACCGCTCCACGCTGTTCCTT | EcoRI |
| JS-pypB6 | CGGAATTCTTTAAACACAATCTCATTTGTCTC | EcoRI |
| GH-flp150f | GCTCTAGAAAAGGATTTTACAACCTGGAA | XbaI |
| GH-flp250f | GCTCTAGACAGTCTTAATCATGATTATCT | XbaI |
| GH-flp500f | GCTCTAGACGTTATACCGGGTCAGTGAGT | XbaI |
| SF-flp1-10 | GGAATTCTTGCGCATTAACTTGTGCTGTAAC | EcoRI |
| JS-flp-KpnI.for | GGGGTACCTATACCGGGTCAGTGAGTAAA | KpnI |
| JS-flp-XbaI.rev | GCTCTAGACCAATCAGGTGGAAATGAAAT | XbaI |
| JS-flp-XhoI.for | CCGCTCGAGATAATAGAAACTCATCTTCTC | XhoI |
| JS-flp-XhoI.rev | CCGCTCGAGAATTCTTTCCTTGTTAAATGT | XhoI |
| JS-pypB.for | GCTCTAGAGGCCTCACTATACCTCAAGCT | XbaI |
| JS-rcpA.rev | CGGGATCCCTTGTTTAGGATTAATTGCAT | BamHI |
| JS-flp1.rev | CGGAATTCTTATTTAACCATGTCTTTTAT | EcoRI |
| JS-flp1 | CGGAATTCAGTCCGTTATACCGGGTCAGTGAG | EcoRI |
| SF-flp1-RT1 | TTACGTTACAGCACAAGTTAATGCG | |
| SF-flp1-RT2 | ATGTTTGTCATGGATGTATCGACG | |
| JS-tadV1 | TAAGGCCAGCGCATTGATAGCGAT | |
| JS-tadV2 | TCTCTATACAGCGATGATGGGCGG | |
| JS-rcpC1 | GCTCGCGATTCAATTCGGTTGAGT | |
| JS-rcpC2 | CCACCATCGATGGCGAAGATGATA | |
| JS-rcpA1 | TCATAGTCTGCGATAGCTGCGGCA | |
| JS-rcpA2 | GTTCTTCCGCAATGCAGAGACACG | |
| JS-tadZ1 | TACACACCAACAATCTCGCGGGGT | |
| JS-tadZ2 | CGAGATAACCGGCAGGTAACTCGT | |
| JS-tadA1 | CGGCGAGAGGTTAAGGTTATCATC | |
| JS-tadA2 | ATTTAAACCCGCTAAAGAACGTGG | |
| JS-tadB1 | GTAAGATAACCATTCGTTAAGCGT | |
| JS-tadB2 | GATCCTATCACCATAGAGTCAATG | |
| JS-tadC1 | GACTGAACACAGGCAGCTGTTATA | |
| JS-tadC2 | CTGCAGCGCACTCCAATATAGTAT | |
| JS-tadD1 | GTGAGCTTCTGCACTATCAGGTGA | |
| JS-tadD2 | TGACCGCTACAGTGATGCCGTAAG | |
| JS-tadE1 | TTAGCAGTCTTGGTGGCCTCCGAA | |
| JS-tadE2 | AGATGATAACAACCTGGCACTCGC | |
| JS-tadF1 | TTACTCGCTCTCGAACGATCCCTG | |
| JS-tadF2 | ATGAGTGACTTATCACCCTATGGA | |
| JS-tadG1 | CGATGGTCAGTGCTAATGTTGCTT | |
| JS-flp.dig | TTTAACCATGTCTTTTATGTT | |
| JS flp2 | GCTCTAGATTAAGCGTAGTCTGGGACGTCGTATGGGTATTTAACCATGTCTTTTATGTTTGTCATGGATGTATC | XbaI |
| JS-alkB2.for | CCCGTCCAGCACGACGAAAGGTTA | |
| KR-cpxA1 | CCGCTCGAGATGCTGGAGCAACACATTGAG | XhoI |
| GH-cpx9 | GAAGATCTGCCCGATAAAGTTACGCACCAT | BglII |
a
Restriction sites are underlined.