FIG. 4.

In vitro phosphorylation of putative StkP substrates. Recombinant His-DivIVA, His-Spr0334, His-PpaC, and His-RpoA were incubated with or without StkP-KD in kinase buffer as described in Materials and Methods. Proteins were separated by SDS-PAGE, electrotransferred to a PVDF membrane, and probed with anti-pThr antibody to detect phosphorylation (anti-pThr) or with anti-His antibody to detect proteins (anti-His tag). The white arrows indicate phosphorylated DivIVA and spr0334. The black arrows on the right indicate the positions of the recombinant proteins.