TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Other designation | Description | Source or reference |
|---|---|---|---|
| E. coli strains | |||
| JM109 | endA1 recA1 gyrA96 thi hsdR17(rK− mK+) relA1 supE44 Δ(lac-proAB) [F′ traD36 proAB lacIqZΔM15] | Promega | |
| BL21(DE3) | F−ompT hsdSB(rB− mB−) gal dcm (DE3) | Novagen | |
| S. pneumoniae strains | |||
| Cp1015 | Sp1 | Rx derivative, str1 hexA | 34 |
| Cp1015ΔstkP | Sp10 | Cp1015 ΔstkP; Cmr | 35 |
| Cp1015stkPK42R | Sp19 | Cp1015, stkP(K42R)-His tag, Cmr, allelic exchange mutant | This study |
| Cp1015stkP-KDtm (stkPΔ372-659) | Sp25 | Cp1015, stkP::kdtm-His tag, Cmr, allelic exchange mutant | 37 |
| D39 | Sp6 | Serotype 2, wild type | 2 |
| Plasmids | |||
| pBluescript II SK+ | Ampr | Stratagene | |
| pEVP3 | Cmr | 7 | |
| pBSK-StkPK42RCm | pBluescript II SK+ derivative used for generation of strain Sp19 by allelic exchange; Ampr Cmr | This study | |
| pEXstkP-T | pET28b derivative used for expression of rStkP-KD; Kanr | 35 | |
| pETPhos | pET derivative used for expression of DivIVA, spr0334, PpaC, and RpoA; Ampr | 5 |