TABLE 2.

Primers used in this study

Primer	Sequence (5′ to 3′)a	Use
CAT 1	CGCGGATCCGAAAATTTGTTTGATTTTTAA	Selection marker
CAT 2	GCTCTAGAGGGTTCC GACCGTCAACGTCAA	Selection marker
STK-f	CGCGAATTCATGATCCAAATCGGCAA	Allelic exchange
STK-r	CGCGGATCCTTAGTGATGGTGATGGTGATGGTGATGGTGATGAGGAGTAGCTGAAGTTGTTTTAGGT	Allelic exchange
X-f	GCTCTAGAGAGTCCAGATTTGTGATA	Allelic exchange
X2-r	TGCCCGCGGGCGTTGTTAATTTATTTA	Allelic exchange
LN89/spr1505-F	CGGCCATATGCCAATTACATCATTAG	DivIVA expression
LN90/spr1505-R	GCCGGAATTCCTACTTCTGGTTCTTCA	DivIVA expression
LN72/spr0334-F	CGGCCATATGAGTAAAAAAAGACGAAATCG	spr0334 expression
LN73/spr0034-R	GCCGGAATTCTTAGTAGTCCAAGTCATC	spr0334 expression
LN1/SPR0215-f	GGCCCGCATATGATCGAGTTTGAAAAA	RpoA expression
LN2/SPR0215-r	CCGCTCGAGTTATTTATCTTTTAATCC	RpoA expression
LN70/ppi-F	GCCGCATATGTCCAAGATTCTAGTATTTGG	PpaC expression
LN71/ppi-R	CGGCGGATCCTTACGCATTAAAGCTTTCAG	PpaC expression

^aRestriction sites are underlined.