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. 2010 May 14;192(14):3608–3619. doi: 10.1128/JB.00345-10

FIG. 9.

FIG. 9.

Germination of heat-activated B. subtilis spores with either normal levels of the GerA receptor (A) or elevated GerA receptor levels (B). Heat-activated (75°C) spores of strains PS533 (normal GerA levels) and PS3476 (≥10-fold GerA receptor levels) were germinated at 30°C in HEPES buffer with various l-valine concentrations, and the percentages of germination of individual spores were determined as described in Materials and Methods using method B. The symbols used (and the percentage of spores that had germinated in 90 min and total number of spores analyzed) are as follows: panel A, ○, 10 mM l-valine (98% of 381 spores), •, 3 mM l-valine (84% of 454 spores), ▵, 1 mM l-valine (44% of 453 spores), and ▴, 0.5 mM l-valine (20% of 433 spores); and panel B, ○, 10 mM l-valine (100% of 483 spores), •, 3 mM l-valine (98% of 423 spores), ▵, 1 mM l-valine (84% of 532 spores), ▴, 0.5 mM l-valine (48% of 448 spores), and □, 0.2 mM l-valine (22% of 441 spores). There was ≤1% germination of the spores used in this figure in the absence of l-valine. Note that (i) the values given on the vertical axis are the percentage of spores that germinated in a particular 5- or 10-min time period in the experiment and (ii) the latter values have been normalized to the total number of spores that germinated in the complete experimental period.